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Sunday, July 26, 2020 | History

2 edition of Aspects of the analysis of bile acids. found in the catalog.

Aspects of the analysis of bile acids.

Theresa O"Reilly

Aspects of the analysis of bile acids.

by Theresa O"Reilly

  • 182 Want to read
  • 9 Currently reading

Published .
Written in English


Edition Notes

Thesis (M. Sc.)--The Queen"s University of Belfast, 1983.

The Physical Object
Pagination1 v
ID Numbers
Open LibraryOL20868571M

Bile acids as characteristic compounds of human and animal bile are major endogenous products of cholesterol and play very important roles in cholesterol homeostasis, lipid absorption and production of bile flow. At present, bile acids are useful biomarkers and signaling molecules for the diagnosis or treatment of many diseases in clinic. While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also required for proper uptake of nutrients in the small intestine. Our Total Bile Acid Assay Kit provides a convenient well plate-based method to measure the total bile acid content in a variety of sample types. These assays are based on an enzyme driven reaction in which bile acids are.

Full Text Analysis of human C24 bile acids metabolome in serum and urine based on enzyme digestion of conjugated bile acids and LC-MS determination of unconjugated bile acids by Zhu, Pingping and Zhang, Jian and Chen, Yujie and Yin, Shanshan and Su, Mingming and Xie, Guoxiang and Brouwer, Kim L. R and Liu, Changxiao and Lan, Ke and Jia, Wei. Objectives. We determined the serum bile acid (BA) composition in patients with liver diseases and healthy volunteers to investigate the relationship between the etiologies of liver disease and BA metabolism. Material and Methods. Sera from patients with liver diseases and 46 healthy volunteers were obtained. The serum concentrations of the 16 different BAs were determined according to Cited by:

The excretion of bile acids into the biliary canaliculi sets up an osmotic gradient, which draws water into bile and drives bile salt-dependent bile flow. Once in the intestine, conjugated bile acids are reabsorbed by active transport receptor-mediated mechanisms in the ileum, which is greater than 90% efficient. called C 27 bile the terminal OH group is oxidized to a carboxyl group, the result is a C 27 bile acid, a class known as cholestanoic acids. (These are also called ancient bile acids.) If the side chain undergoes oxidative carboxylation to form a C5 side chain, the result is a modern bile acid and the class contains cholanoic l bile alcohols also include C 26, C 25, and.


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Aspects of the analysis of bile acids by Theresa O"Reilly Download PDF EPUB FB2

An LC/MS/MS-based method was developed for the determination of individual bile acids (BA) and their conjugates in porcine bile samples.

The Cbased solid-phase extraction (SPE) procedure was Author: Kirti Rani. BSEP mainly transports monovalent conjugated bile acids and is a high-affinity taurocholate transporter.

It is also responsible for the high-affinity transport of glycochenodeoxycholate, glycocholate and taurochenodeoxycholate, which are the major bile salt species present in human bile (Table ) (Hofmann, ).The substrate specificities are in line with a near-total absence of these bile.

Bile acids are hydroxylated steroids, synthesized in the liver from cholesterol. Peroxisomal enzymes assist in the hepatic biosynthesis of BAs. Bile acids are normally conjugated in the liver to the amino acids, Gly and Tau, or sulfate.

The active transport of BAs across canalicular membranes of hepatocytes is a primary driving force for bile flow. This book examines the biological aspects of bile acid analysis and their clinical applications, providing readers with an overview of cutting-edge discussions on Bile Acid research and diseases.

It begins with detailed discussions on the biological aspects of bile acid, with chapters examining bile acid from various perspectives, from its. The composition of circulating bile acids in humans is a complex mixture of primary and secondary bile acids. In humans, primary bile acids are chenodeoxycholic acid (CDCA) and cholic acid (CA), each secreted into bile in mostly N-acyl amidated form, i.e., conjugated with glycine or taurine in an amide linkage (1–3).In the distal intestine, bile acids are modified by bacterial by:   Most bile acids (roughly 95%) are then reabsorbed further along the small intestine (in the ileum), and then transported via the portal vein back to the liver.

But the importance of bile acids in the small intestine does not end there – there is much more to. Bile acids are steroids with a pentanoic acid substituent at C They are the terminal products of Aspects of the analysis of bile acids.

book excretion, and play critical physiological roles in human and animals. Bile acids are easy to detect but difficult to identify by using mass spectrometry due to their poly-ring structure and various hydroxylation by: Bile Acids in Gastroenterology PDF Free Download E-BOOK DESCRIPTION This book examines the biological aspects of bile acid analysis and their clinical applications, providing readers with an overview of cutting-edge discussions on Bile Acid research and diseases.

This book examines the biological aspects of bile acid analysis and their clinical applications, providing readers with an overview of cutting-edge discussions on Bile Acid research and diseases. It begins with detailed discussions on the biological aspects of bile acid, with chapters examining.

Bile acids (BAs) are useful biomarkers for the diagnosis of many diseases. The pathologies related to bile acid synthesis are often expressed in the first years of life and may lead to serious liver injury.

Here we present a sensitive and rapid method for the analysis of the main 14 bile acids in human serum by liquid chromatography-tandem mass by: This book examines the biological aspects of bile acid analysis and their clinical applications, providing readers with an overview of cutting-edge discussions on Bile Acid research and diseases.

It begins with detailed discussions on the biological aspects of bile acid, with chapters examining bile acid from various perspectives, from its Manufacturer: Springer.

b The bile acid described is a tetrahydroxy bile acid (3 ␣,7 ␣,12 ␣,tetrahydroxy). at Univ of California - San Diego Serials/Biomed Libon August 8, Downloaded from. Bile Acids in Gastroenterology: Basic and Clinical [Tazuma, Susumu, Takikawa, Hajime] on *FREE* shipping on qualifying offers.

Bile Acids in Gastroenterology: Basic and Clinical. Damage to the liver occurs from multiple effects of the various retained biliary constituents, including various lipids, toxins, and bile acids.

Therefore impairments in bile flow and secretion – cholestasis – particularly in the infant liver, drive the development and progression of liver disease. The complex nature of the bile acids exist in the sample is the key factor determines the analytical method to be used.

The bile acids are rather complex mixture and quantification of bile acids is needed. Simple analytical quantification methods have limitations, so time-consuming LC-MS quantification method is needed for bile acids analysis.

The second edition of this book has drawn heavily on the first edition but a huge amount of research on steroid analysis has been published over the last 15 years. As a result, the Editors decided to let the first edition of this book stand on its own and direct readers interested in pre Quantitative Bile Acids Analysis.

The Duke Proteomics and Metabolomics Shared Resource utilizes the Biocrates Bile Acids Kit to analyze endogenous bile acids, including 19 conjugated and unconjugated, primary and secondary bile acids. Bile acids have many biological functions, including biomarkers for liver function, signaling molecules with hormone-like functions and biomarkers for gut.

BioAssay Systems' Bile Acid Assay Kit (EFBA) provides a fluorimetric means to measure total bile acids. Fluorescence intensity (/ nm) is linear to the bile acid concentration in the sample. Samples: serum, plasma, urine and other biological samples.

Detection range: 1 - µM bile acids. Bile acids are steroid acids found predominantly in the bile of mammals and other e bile acids are synthesized in the liver.

Bile acids are conjugated with taurine or glycine residues to give anions called bile salts. Primary bile acids are those synthesized by the ary bile acids result from bacterial actions in the humans, taurocholic acid and.

Get this from a library. Bile acids in gastroenterology: basic and clinical. [Susumu Tazuma; Hajime Takikawa;] -- This book examines the biological aspects of bile acid analysis and their clinical applications, providing readers with an overview of cutting-edge discussions on.

Studies in Analytical Chemistry, Volume 5: Quantitative Analysis of Steroids covers the pharmaceutical aspects of the analysis of steroid hormones.

This book is divided into nine chapters that examine the biological-clinical analysis of other important groups of steroids, including sterols, vitamin D, bile acids, cardiac glycosides, and sapogenins.Bile acids are a family of amphipathic compounds predominantly known for their role in solubilizing and absorbing hydrophobic compounds (including liposoluble vitamins) in the intestine.

Bile acids also are key signaling molecules and inflammatory agents that activate transcriptional factors and cell signaling pathways that regulate lipid, glucose, and energy metabolism in various human Cited by: 1.Get this from a library!

The Bile Acids: Chemistry, Physiology, and Metabolism: Volume 4: Methods and Applications. [Kenneth D R Setchell; David Kritchevsky; Padmanabhan P Nair] -- Over a decade has elapsed since the last volume in this series was published.

At that time we considered that we had comprehensively covered all aspects relating to bile acid chemistry and.